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Optical Readout of Gold Nanoparticle-Based DNA Microarrays without Silver Enhancement

Gerhard A. Blab ^*, Laurent Cognet ^*, Stéphane Berciaud ^*, Isabelle Alexandre , Dieter Husar , José Remacle  and Brahim Lounis ^*

^* Centre de Physique Moléculaire Optique et Hertzienne–CNRS UMR 5798 et Université Bordeaux I, 33405 Talence Cedex, France; and Eppendorf Array Technologies, 5000 Namur, Belgium

Correspondence: Address reprint requests and inquiries to Brahim Lounis, E-mail: b.lounis{at}cpmoh.u-bordeaux1.fr.

We present a novel readout scheme for gold nanoparticle-based DNA microarrays relying on "Laser-Induced Scattering around a NanoAbsorber". It provides direct counting of individual nanoparticles present on each array spot and stable signals, without any silver enhancement. Given the detection of nanometer-sized particles, which minimize the steric hindrance, the linear dynamic range of the method is particularly large and well suited for microarray detection.

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