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Viscoelastic Retraction of Single Living Stress Fibers and Its Impact on Cell Shape, Cytoskeletal Organization, and Extracellular Matrix Mechanics

Sanjay Kumar ^*, Iva Z. Maxwell , Alexander Heisterkamp , Thomas R. Polte ^*, Tanmay P. Lele ^*, Matthew Salanga , Eric Mazur  and Donald E. Ingber ^*

^* Vascular Biology Program, Departments of Pathology and Surgery, Children's Hospital and Harvard Medical School, Boston, Massachusetts; Department of Physics, Division of Engineering and Applied Sciences, Harvard University, Cambridge, Massachusetts; and Mental Retardation Research Center, Children's Hospital, Boston, Massachusetts

Correspondence: Address reprint requests to Donald E. Ingber, MD, PhD, Vascular Biology Program, Children's Hospital, Karp Family Research Laboratories, 11.127 300 Longwood Ave., Boston, MA 02115-5737. Tel.: 617-919-2223; Fax: 617-730-0230; E-mail: donald.ingber{at}childrens.harvard.edu.

Cells change their form and function by assembling actin stress fibers at their base and exerting traction forces on their extracellular matrix (ECM) adhesions. Individual stress fibers are thought to be actively tensed by the action of actomyosin motors and to function as elastic cables that structurally reinforce the basal portion of the cytoskeleton; however, these principles have not been directly tested in living cells, and their significance for overall cell shape control is poorly understood. Here we combine a laser nanoscissor, traction force microscopy, and fluorescence photobleaching methods to confirm that stress fibers in living cells behave as viscoelastic cables that are tensed through the action of actomyosin motors, to quantify their retraction kinetics in situ, and to explore their contribution to overall mechanical stability of the cell and interconnected ECM. These studies reveal that viscoelastic recoil of individual stress fibers after laser severing is partially slowed by inhibition of Rho-associated kinase and virtually abolished by direct inhibition of myosin light chain kinase. Importantly, cells cultured on stiff ECM substrates can tolerate disruption of multiple stress fibers with negligible overall change in cell shape, whereas disruption of a single stress fiber in cells anchored to compliant ECM substrates compromises the entire cellular force balance, induces cytoskeletal rearrangements, and produces ECM retraction many microns away from the site of incision; this results in large-scale changes of cell shape (> 5% elongation). In addition to revealing fundamental insight into the mechanical properties and cell shape contributions of individual stress fibers and confirming that the ECM is effectively a physical extension of the cell and cytoskeleton, the technologies described here offer a novel approach to spatially map the cytoskeletal mechanics of living cells on the nanoscale.

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