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Originally published as Biophys J. BioFAST on February 3, 2006.
doi:10.1529/biophysj.105.072215
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Biophysical Journal 90:3796-3805 (2006)
© 2006 The Biophysical Society

A Master Relation Defines the Nonlinear Viscoelasticity of Single Fibroblasts

Pablo Fernández, Pramod A. Pullarkat and Albrecht Ott

Experimentalphysik I, Physikalisches Institut, Universität Bayreuth, D-95440 Bayreuth, Germany

Correspondence: Address reprint requests to Pablo Fernández, Tel.: 49-921-55-2502; Fax: 49-921-55-2521; E-mail: pablo{at}ep1.uni-bayreuth.de.

Cell mechanical functions such as locomotion, contraction, and division are controlled by the cytoskeleton, a dynamic biopolymer network whose mechanical properties remain poorly understood. We perform single-cell uniaxial stretching experiments on 3T3 fibroblasts. By superimposing small amplitude oscillations on a mechanically prestressed cell, we find a transition from linear viscoelastic behavior to power law stress stiffening. Data from different cells over several stress decades can be uniquely scaled to obtain a master relation between the viscoelastic moduli and the average force. Remarkably, this relation holds independently of deformation history, adhesion biochemistry, and intensity of active contraction. In particular, it is irrelevant whether force is actively generated by the cell or externally imposed by stretching. We propose that the master relation reflects the mechanical behavior of the force-bearing actin cytoskeleton, in agreement with stress stiffening known from semiflexible filament networks.




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