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Recognition Imaging with a DNA Aptamer

Liyun Lin ^* , Hongda Wang , Yan Liu  , Hao Yan   and Stuart Lindsay ^*  

^* Department of Physics, Department of Chemistry and Biochemistry, and Biodesign Institute, Arizona State University, Tempe, Arizona

Correspondence: Address reprint requests to Stuart Lindsay, E-mail: stuart.lindsay{at}asu.edu.

We have used a DNA-aptamer tethered to an atomic force microscope probe to carry out recognition imaging of IgE molecules attached to a mica substrate. The recognition was efficient (90%) and specific, being blocked by injection of IgE molecules in solution, and not being interfered with by high concentrations of a second protein. The signal/noise ratio of the recognition signal was better than that obtained with antibodies, despite the fact that the average force required to break the aptamer-protein bonds was somewhat smaller.