help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on March 24, 2006.
doi:10.1529/biophysj.105.079905
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
biophysj.105.079905v1
90/12/4317    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Skoge, M. L.
Right arrow Articles by Wingreen, N. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Skoge, M. L.
Right arrow Articles by Wingreen, N. S.
Biophysical Journal 90:4317-4326 (2006)
© 2006 The Biophysical Society

Receptor-Receptor Coupling in Bacterial Chemotaxis: Evidence for Strongly Coupled Clusters

Monica L. Skoge *, Robert G. Endres {dagger} {ddagger} and Ned S. Wingreen {dagger}

* Department of Physics and {dagger} Department of Molecular Biology, Princeton University, Princeton, New Jersey; and {ddagger} NEC Laboratories America, Princeton, New Jersey

Correspondence: Address reprint requests to Ned S. Wingreen, Tel.: 609-258-8476; E-mail: wingreen{at}princeton.edu.

Receptor coupling is believed to explain the high sensitivity of the Escherichia coli chemotaxis network to small changes in levels of chemoattractant. We compare in detail the activity response of coupled two-state receptors for different models of receptor coupling: weakly-coupled extended one-dimensional and two-dimensional lattice models and the Monod-Wyman-Changeux model of isolated strongly-coupled clusters. We identify features in recent data that distinguish between the models. Specifically, researchers have measured the receptor activity response to steps of chemoattractant for a variety of engineered E. coli strains using in vivo fluorescence resonance energy transfer. We find that the fluorescence resonance energy transfer results for wild-type and for a low-activity mutant are inconsistent with the lattice models of receptor coupling, but consistent with the Monod-Wyman-Changeux model of receptor coupling, suggesting that receptors form isolated strongly-coupled clusters.




This article has been cited by other articles:


Home page
 Biophys. JHome page
B. A. Mello and Y. Tu
Effects of Adaptation in Maintaining High Sensitivity over a Wide Range of Backgrounds for Escherichia coli Chemotaxis
Biophys. J., April 1, 2007; 92(7): 2329 - 2337.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. G. Endres and N. S. Wingreen
Precise adaptation in bacterial chemotaxis through "assistance neighborhoods"
PNAS, August 29, 2006; 103(35): 13040 - 13044.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2006 by the Biophysical Society.