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Originally published as Biophys J. BioFAST on March 24, 2006.
doi:10.1529/biophysj.105.080283
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Biophysical Journal 90:4428-4436 (2006)
© 2006 The Biophysical Society

Closed-Loop Miscibility Gap and Quantitative Tie-Lines in Ternary Membranes Containing Diphytanoyl PC

Sarah L. Veatch *, Klaus Gawrisch {dagger} and Sarah L. Keller *

* Departments of Chemistry and Physics, University of Washington, Seattle, Washington; and {dagger} National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Bethesda, Maryland

Correspondence: Address reprint requests to Sarah L. Keller, Dept. of Chemistry, University of Washington, Seattle, WA. E-mail: slkeller{at}chem.washington.edu.

Vesicles containing ternary mixtures of diphytanoylphosphatidylcholine, dipalmitoylphosphatidylcholine (DPPC), and cholesterol produce coexisting liquid phases over an unusually large range of temperature and composition. Liquid domains persist well above the DPPC chain melting temperature (41°C), resulting in a closed-loop miscibility gap bounded by two critical points at fixed temperature. Quantitative tie-lines are determined directly from 2H NMR spectra using a novel analysis, and are found to connect a liquid-disordered phase rich in diphytanoyl PC with a liquid-ordered phase rich in DPPC. The direction of the tie-lines implies that binary DPPC/cholesterol mixtures are in one uniform phase above 41°C. All 2H NMR results for tie-lines are verified by independent fluorescence microscopy results.




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