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Originally published as Biophys J. BioFAST on December 9, 2005.
doi:10.1529/biophysj.105.077974
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Biophysical Journal 90:L24-L26 (2006)
© 2006 The Biophysical Society

Measuring Limits of Telomere Movement on Nuclear Envelope

Angelo Rosa *, John H. Maddocks *, Frank R. Neumann {dagger}, Susan M. Gasser {dagger} and Andrzej Stasiak {ddagger}

* Laboratory for Computation and Visualization in Mathematics and Mechanics, Ecole Polytechnique Fédérale de Lausanne, CH-1015 Lausanne, Switzerland; {dagger} Friedrich Miescher Institute for Biomedical Research, CH-4058 Basel, Switzerland; and {ddagger} Department of Biology and Medicine, Université de Lausanne, CH-1015 Lausanne, Switzerland

Correspondence: Address reprint requests and inquiries to Angelo Rosa, Tel.: 49-0351-871-1808; Fax: 49-0351-871-1999; E-mail: rosa{at}mpipks-dresden.mpg.de.

The dynamic behavior of the decondensed chromatin can be monitored by real-time fluorescence confocal microscopy. It can be observed that different chromosomal sites enjoy different degrees of freedom during a certain period, exploring larger or smaller portions of nuclear volume. Here we measure the accessible surface for two chromosomal sites (yeast telomeres Tel3R and Tel6R) that both exhibit strong preferential association with the nuclear membrane in galactose-containing media, but differ significantly in gene activity. Telomere Tel6R, which harbors an inducible gene with high levels of transcription, explores a much larger surface than the telomere Tel3R, which is adjacent to inactive chromatin. Thus, our results distinguish two perinuclear movements characteristic of different transcriptional state, allowing for a better understanding of the correlation between activity of genes and chromatin dynamics.






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Copyright © 2006 by the Biophysical Society.