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Effect of Average Phospholipid Curvature on Supported Bilayer Formation on Glass by Vesicle Fusion

Chiho Hamai ^*, Tinglu Yang , Sho Kataoka , Paul S. Cremer  and Siegfried M. Musser ^*

^* Department of Molecular and Cellular Medicine, The Texas A&M University System Health Science Center, College Station, Texas 77843; and Department of Chemistry, Texas A&M University, College Station, Texas 77842

Correspondence: Address reprint requests to Siegfried M. Musser, Tel.: 979-862-4128; Fax: 979-847-9481; E-mail: smusser{at}tamu.edu.

The adsorption of large unilamellar vesicles composed of various combinations of phosphatidylcholine, phosphatidylethanolamine (PE), monomethyl PE, and dimethyl PE (PE-Me₂) onto a glass surface was studied using fluorescence microscopy. The average lipid geometry within the vesicles, described mathematically by the average intrinsic curvature, C_0,ave, was methodically altered by changing the lipid ratios to determine the effect of intrinsic curvature on the ability of vesicles to rupture and form a supported lipid bilayer. We show that the ability of vesicles to create fluid planar bilayers is dependent on C_0,ave and independent of the identity of the component lipids. When the C_0,ave was –0.1 nm^–1, the vesicles readily formed supported lipid bilayers with almost full mobility. In contrast, when the C_0,ave ranged from –0.2 to –0.3 nm^–1, the adsorbed vesicles remained intact upon the surface. The results indicate that the average shape of lipid molecules within a vesicle (C_0,ave) is essential for determining kinetically viable reactions that are responsible for global geometric changes.

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