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Originally published as Biophys J. BioFAST on April 7, 2006.
doi:10.1529/biophysj.105.079046
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Biophysical Journal 91:179-188 (2006)
© 2006 The Biophysical Society

Clustering of Cyclic-Nucleotide-Gated Channels in Olfactory Cilia

Richard J. Flannery *, Donald A. French {dagger} and Steven J. Kleene *

* Department of Cell Biology, Neurobiology, and Anatomy, and {dagger} Department of Mathematical Sciences, University of Cincinnati, Cincinnati, Ohio

Correspondence: Address reprint requests to Steven J. Kleene, Dept. of Cell Biology, Neurobiology, and Anatomy, University of Cincinnati, PO Box 670667, Cincinnati, OH 45267-0667. Tel.: 513-558-6099; Fax: 513-558-2727; E-mail: steve{at}syrano.acb.uc.edu.

Olfactory cilia contain the known components of olfactory signal transduction, including a high density of cyclic-nucleotide-gated (CNG) channels. CNG channels play an important role in mediating odor detection. The channels are activated by cAMP, which is formed by a G-protein-coupled transduction cascade. Frog olfactory cilia are 25–200 µm in length, so the spatial distribution of CNG channels along the length should be important in determining the sensitivity of odor detection. We have recorded from excised cilia and modeled diffusion of cAMP into a cilium to determine the spatial distribution of the CNG channels along the ciliary length. The proximal segment, which in frog is the first 20% of the cilium, appears to express a small fraction of the CNG channels, whereas the distal segment contains the majority, mostly clustered in one region.




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