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Originally published as Biophys J. BioFAST on September 22, 2006.
doi:10.1529/biophysj.106.089771
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Biophysical Journal 91:4598-4604 (2006)
© 2006 The Biophysical Society

Single Nanoparticle Photothermal Tracking (SNaPT) of 5-nm Gold Beads in Live Cells

David Lasne *, Gerhard A. Blab *, Stéphane Berciaud *, Martin Heine {dagger}, Laurent Groc {dagger}, Daniel Choquet {dagger}, Laurent Cognet * and Brahim Lounis *

* Centre de Physique Moléculaire Optique et Hertzienne, CNRS (UMR 5798) and Université Bordeaux 1, Talence Cedex, France; and {dagger} Physiologie Cellulaire de la Synapse, CNRS (UMR 5091) and Université Bordeaux 2, Institut François Magendie, Bordeaux Cedex, France

Correspondence: Address reprint requests to Brahim Lounis, E-mail: b.lounis{at}cpmoh.u-bordeaux1.fr.

Tracking individual nano-objects in live cells during arbitrary long times is a ubiquitous need in modern biology. We present here a method for tracking individual 5-nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against single fluorescent molecule tracking in live COS7 cells on subsecond timescales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. Single Nanoparticle Photothermal Tracking has the unique potential to record arbitrary long trajectory of membrane proteins using nonfluorescent nanometer-sized labels.




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[Abstract] [Full Text] [PDF]


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