This Article Full Text Full Text (PDF) Supplement All Versions of this Article: biophysj.106.085118v1 91/4/1357    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Triba, M. N. Articles by Warschawski, D. E. Search for Related Content PubMed PubMed Citation Articles by Triba, M. N. Articles by Warschawski, D. E.

Effects of Lipid Chain Length and Unsaturation on Bicelles Stability. A Phosphorus NMR Study

Unité Mixte de Recherche No. 7099, Centre National de la Recherche Scientifique, Institut de Biologie Physico-Chimique, Paris, France

Correspondence: Address reprint requests to Dror E. Warschawski, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France. Tel.: 33-1-58-41-51-11; Fax: 33-1-58-41-50-24; E-mail: dror.warschawski{at}ibpc.fr.

Most studies reported until now on the magnetically alignable system formed by the binary mixtures of long- and short-chain lipids were based on the mixture of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (D14PC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (D6PC) lipids. We have recently shown that a large part of the phase diagrams of this lipid mixture could be understood by taking into account the partial miscibility between the long-chain lipids and the short-chain lipids when the sample was heated above the melting transition temperature (T_m) of the long-chain lipids. In this work, we show by modifying the chain length of either one of the two lipids that it is possible to control their miscibility and thus the intervals of temperature and composition where spontaneous alignment is observed in a magnetic field. By using ³¹P NMR, we demonstrate that the very special properties of such binary lipid mixtures are correlated with the propensity for short-chain lipids to diffuse into the bilayer regions. We also show that lipid mixtures with comparable properties can be formed with unsaturated lipids such as 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC).

This article has been cited by other articles:

				J. Pan, S. Tristram-Nagle, N. Kucerka, and J. F. Nagle Temperature Dependence of Structure, Bending Rigidity, and Bilayer Interactions of Dioleoylphosphatidylcholine Bilayers Biophys. J., January 1, 2008; 94(1): 117 - 124. [Abstract] [Full Text] [PDF]