This Article Full Text Full Text (PDF) All Versions of this Article: biophysj.106.082420v1 91/5/1960    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by McNamee, C. E. Articles by Higashitani, K. Search for Related Content PubMed PubMed Citation Articles by McNamee, C. E. Articles by Higashitani, K.

Atomic Force Microscopy Study of the Specific Adhesion between a Colloid Particle and a Living Melanoma Cell: Effect of the Charge and the Hydrophobicity of the Particle Surface

Department of Chemical Engineering, Kyoto University-Katsura, Kyoto 615-8510 Japan

Correspondence: Address reprint requests to Cathy E. McNamee, Dept. of Chemical Engineering, Kyoto University-Katsura, Nishikyo-ku, Kyoto 615-8510, Japan. Tel: 81-075-383-2692; Fax: 81-075-383-2692; E-mail: cathymcn{at}cheme.kyoto-u.ac.jp.

We investigated the effect of the charge and the hydrophobicity of drug delivery system (DDS) carriers on their specificity to living malignant melanoma B16F10 cells with the atomic force microscope. To model various nanoparticle DDS carriers, we used silica particles that were modified with silane coupling agents. We then measured the compression and decompression forces between the modified colloid probes and the living B16F10 cell in a physiological buffer as a function of their separation distances. The maximum adhesive force on decompression was related to the strength of the specificity of the DDS to the malignant cell. A comparison of the average maximum adhesive force of each functionality group surprisingly showed that negatively charged surfaces and hydrophobic modified surfaces all had similar low values. Additionally, we saw the unexpected result that there was no observable dependence on the degree of hydrophobicity of the probe surface to a B16F10 cell. Only the positively charged particle gave a strong adhesive force with the B16F10 cell. This indicated that DDS carriers with positive charges appeared to have the highest affinity for malignant melanoma cells and that the use of hydrophobic materials unexpectedly did not improve their affinity.

This article has been cited by other articles:

				A. P. Gunning, S. Chambers, C. Pin, A. L. Man, V. J. Morris, and C. Nicoletti Mapping specific adhesive interactions on living human intestinal epithelial cells with atomic force microscopy FASEB J, July 1, 2008; 22(7): 2331 - 2339. [Abstract] [Full Text] [PDF]

				C. E. McNamee, S. Yamamoto, and K. Higashitani Effect of the Physicochemical Properties of Poly(Ethylene Glycol) Brushes on their Binding to Cells Biophys. J., July 1, 2007; 93(1): 324 - 334. [Abstract] [Full Text] [PDF]