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Originally published as Biophys J. BioFAST on August 4, 2006.
doi:10.1529/biophysj.106.091439
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Biophysical Journal 91:L75-L77 (2006)
© 2006 The Biophysical Society

Curvature Elasticity and Refolding of OmpA in Large Unilamellar Vesicles

Cosmin L. Pocanschi *, Geetika J. Patel *, Derek Marsh {dagger} and Jörg H. Kleinschmidt *

* Universität Konstanz, Fachbereich Biologie, 78547 Konstanz, Germany; and {dagger} Max-Planck-Institut für biophysikalische Chemie, Abteilung Spektroskopie, 37070 Göttingen, Germany

Correspondence: Address reprint requests and inquiries to Jörg Helmut Kleinschmidt, E-mail: joerg.helmut.kleinschmidt{at}uni-konstanz.de; or to Derek Marsh, E-mail: dmarsh{at}gwdg.de.

The stability of OmpA in large unilamellar vesicles of dilauroyl phosphatidylcholine was studied using different concentrations of urea. The effective energy of unfolding, as determined from refolding experiments, is greater than that for small sonicated unilamellar vesicles by an amount that is compatible with estimates of the elastic energy of highly curved vesicles. The on-rate for refolding and insertion is slower for large unilamellar vesicles than for small unilamellar vesicles, which indicates a contribution of vesicle strain also to the free energy of the transition state.




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