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Originally published as Biophys J. BioFAST on March 16, 2007.
doi:10.1529/biophysj.107.105320
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Biophysical Journal 92:L89-L91 (2007)
© 2007 The Biophysical Society

Multiplex SNP Discrimination

Daniel J. Fish *, M. Todd Horne * {dagger}, Robert P. Searles {ddagger}, Greg P. Brewood * and Albert S. Benight * {dagger}

* Portland Bioscience, Portland, Oregon 97201; {dagger} Department of Chemistry and Department of Physics, Portland State University, Portland, Oregon 97207; and {ddagger} Spotted Microarray Core, Oregon Health and Science University, Beaverton, Oregon 97006

Correspondence: Address reprint requests and inquiries to Daniel Fish, E-mail: djf{at}pdxbio.com.

Multiplex hybridization reactions of perfectly matched duplexes and duplexes containing a single basepair mismatch (SNPs) were investigated on DNA microarrays. Effects of duplex length, G-C percentage, and relative position of the SNP on duplex hybridization and SNP resolution were determined. Our theoretical model of multiplex hybridization accurately predicts observed results and implicates target concentration as a critical variable in multiplex SNP detection.




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Copyright © 2007 by the Biophysical Society.