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Originally published as Biophys J. BioFAST on March 9, 2007.
doi:10.1529/biophysj.106.092114
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Biophysical Journal 92:4054-4063 (2007)
© 2007 The Biophysical Society

C-Phycocyanin Hydration Water Dynamics in the Presence of Trehalose: An Incoherent Elastic Neutron Scattering Study at Different Energy Resolutions

Frank Gabel * and Marie-Claire Bellissent-Funel {dagger}

* Institut de Biologie Structurale Jean-Pierre Ebel, Commissariat à l'Énergie Atomique-Centre National de la Recherche Scientifique-Université Joseph Fourier, Grenoble, France; and {dagger} Laboratoire Léon Brillouin, Commissariat à l'Énergie Atomique Saclay, Gif-sur-Yvette, France

Correspondence: Address reprint requests to Frank Gabel, Institut de Biologie Structurale, 41 rue Jules Horowitz, 38027 Grenoble, France. Tel.: 33-438789573; E-mail: frank.gabel{at}ibs.fr.

We present a study of C-phycocyanin hydration water dynamics in the presence of trehalose by incoherent elastic neutron scattering. By combining data from two backscattering spectrometers with a 10-fold difference in energy resolution we extract a scattering law S(Q,{omega}) from the Q-dependence of the elastic intensities without sampling the quasielastic range. The hydration water is described by two dynamically different populations—one diffusing inside a sphere and the other diffusing quasifreely—with a population ratio that depends on temperature. The scattering law derived describes the experimental data from both instruments excellently over a large temperature range (235–320 K). The effective diffusion coefficient extracted is reduced by a factor of 10–15 with respect to bulk water at corresponding temperatures. Our approach demonstrates the benefits and the efficiency of using different energy resolutions in incoherent elastic neutron scattering over a large angular range for the study of biological macromolecules and hydration water.




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