help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Originally published as Biophys J. BioFAST on July 27, 2007.
doi:10.1529/biophysj.107.112201
OPEN ACCESS ARTICLE
This Article
Free via Open Access: OA
Right arrow OA Full Text
Right arrow Full Text (PDF)
Right arrowOA All Versions of this Article:
biophysj.107.112201v1
93/9/3285    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Egner, A.
Right arrow Articles by Hell, S. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Egner, A.
Right arrow Articles by Hell, S. W.
Biophysical Journal 93:3285-3290 (2007)
© 2007 The Biophysical Society

This is an Open Access article distributed under the terms of the Creative Commons-Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/2.0/), which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Fluorescence Nanoscopy in Whole Cells by Asynchronous Localization of Photoswitching Emitters

Alexander Egner *, Claudia Geisler *, Claas von Middendorff *, Hannes Bock *, Dirk Wenzel {dagger}, Rebecca Medda *, Martin Andresen *, Andre C. Stiel *, Stefan Jakobs *, Christian Eggeling *, Andreas Schönle * and Stefan W. Hell *

* Department of NanoBiophotonics, {dagger} Department of Neurobiology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany

Correspondence: Address reprint requests to A. Egner, Tel.: 49-0-551-201-2500; E-mail: aegner{at}gwdg.de; or to S. Hell, E-mail: shell{at}gwdg.de.

We demonstrate nanoscale resolution in far-field fluorescence microscopy using reversible photoswitching and localization of individual fluorophores at comparatively fast recording speeds and from the interior of intact cells. These advancements have become possible by asynchronously recording the photon bursts of individual molecular switching cycles. We present images from the microtubular network of an intact mammalian cell with a resolution of 40 nm.




This article has been cited by other articles:


Home page
 ScienceHome page
B. Huang, W. Wang, M. Bates, and X. Zhuang
Three-Dimensional Super-Resolution Imaging by Stochastic Optical Reconstruction Microscopy
Science, February 8, 2008; 319(5864): 810 - 813.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Shroff, C. G. Galbraith, J. A. Galbraith, H. White, J. Gillette, S. Olenych, M. W. Davidson, and E. Betzig
Dual-color superresolution imaging of genetically expressed probes within individual adhesion complexes
PNAS, December 18, 2007; 104(51): 20308 - 20313.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2007 by the Biophysical Society.