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Clockwise Translocation of Microtubules by Flagellar Inner-Arm Dyneins In Vitro

Kenji Kikushima ^* and Ritsu Kamiya ^* 

^* Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan; and CREST, Japan Science and Technology Corporation, Kawaguchi, Japan

Correspondence: Address reprint requests to Dr. Ritsu Kamiya, Dept. of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan. Tel.: 81-3-5841-4426; Fax: 81-3-5841-4632; E-mail: kamiyar{at}biol.s.u-tokyo.ac.jp.

Cilia and flagella are equipped with multiple species of dyneins that have diverse motor properties. To assess the properties of various axonemal dyneins of Chlamydomonas, in vitro microtubule translocation by isolated dyneins was examined with and without flow of the medium. With one inner-arm dynein species, dynein c, most microtubules became aligned parallel to the flow and translocated downstream after the onset of flow. When the flow was stopped, the gliding direction was gradually randomized. In contrast, with inner-arm dyneins d and g, microtubules tended to translocate at a shallow right angle to the flow. When the flow was stopped, each microtubule turned to the right, making a curved track. The clockwise translocation was not accompanied by lateral displacement, indicating that these dyneins generate torque that bends the microtubule. The torque generated by these dyneins in the axoneme may modulate the relative orientation between adjacent doublet microtubules and lead to more efficient functioning of total dyneins.