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Osmotically Induced Helix-Coil Transition in Poly(Glutamic Acid)

Christopher B. Stanley ^* and Helmut H. Strey 

^* Department of Polymer Science and Engineering, University of Massachusetts, Amherst, Massachusetts; and Department of Biomedical Engineering, State University of New York, Stony Brook, New York

Correspondence: Address reprint requests to Helmut H. Strey, Dept. of Biomedical Engineering, Center for Biotechnology, Psychology-A Bldg., 3rd Fl., State University of New York, Stony Brook, NY 11794-2580. Tel.: 631-632-1957; Fax: 631-632-8577; E-mail: Helmut.Strey{at}stonybrook.edu.

Protein folding and conformational changes are influenced by protein-water interactions and, as such, the energetics of protein function are necessarily linked to water activity. Here, we have chosen the helix-coil transition in poly(glutamic acid) as a model system to investigate the importance of hydration to protein structure by using the osmotic stress method combined with circular dichroism spectroscopy. Osmotic stress is applied using poly(ethylene glycol), molecular weight of 400, as the osmolyte. The energetics of the helix-coil transition under applied osmotic stress allows us to calculate the change in the number of preferentially included water molecules per residue accompanying the thermally induced conformational change. We find that osmotic stress raises the helix-coil transition temperature by favoring the more compact -helical state over the more hydrated coil state. The contribution of other forces to -helix stability also are explored by varying pH and studying a random copolymer, poly(glutamic acid-r-alanine). In this article, we clearly show the influence of osmotic pressure on the peptide folding equilibrium. Our results suggest that to study protein folding in vitro, the osmotic pressure, in addition to pH and salt concentration, should be controlled to better approximate the crowded environment inside cells.