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Unloaded Speed of Shortening in Voltage-Clamped Intact Skeletal Muscle Fibers from wt, mdx, and Transgenic Minidystrophin Mice Using a Novel High-Speed Acquisition System

O. Friedrich ^*, C. Weber ^*, F. v. Wegner ^*, J. S. Chamberlain  and R. H. A. Fink ^*

^* Medical Biophysics, Department of Systems Physiology, Institute of Physiology and Pathophysiology, Ruprecht-Karls-University, Heidelberg, Germany; and Department of Neurology, University of Washington, Seattle, Washington

Correspondence: Address reprint requests to O. Friedrich, Medical Biophysics, Dept. of Systems Physiology, Institute of Physiology and Pathophysiology, Ruprecht-Karls-University, INF 326, 69120 Heidelberg, Germany. Tel.: 49-6221-54-4143; Fax: 49-6221-54-4123; E-mail: oliver.friedrich{at}physiologie.uni-heidelberg.de.

Skeletal muscle unloaded shortening has been indirectly determined in the past. Here, we present a novel high-speed optical tracking technique that allows recording of unloaded shortening in single intact, voltage-clamped mammalian skeletal muscle fibers with 2-ms time resolution. L-type Ca²⁺ currents were simultaneously recorded. The time course of shortening was biexponential: a fast initial phase, ₁, and a slower successive phase, _2, with activation energies of 59 kJ/mol and 47 kJ/mol. Maximum unloaded shortening speed, v_u,max, was faster than that derived using other techniques, e.g., 14.0 L₀ s^–1 at 30°C. Our technique also allowed direct determination of shortening acceleration. We applied our technique to single fibers from C57 wild-type, dystrophic mdx, and minidystrophin-expressing mice to test whether unloaded shortening was affected in the pathophysiological mechanism of Duchenne muscular dystrophy. v_u,max and a_u,max values were not significantly different in the three strains, whereas ₁ and ₂ were increased in mdx fibers. The results were complemented by myosin heavy and light chain (MLC) determinations that showed the same myosin heavy chain IIA profiles in the interossei muscles from the different strains. In mdx muscle, MLC-1f was significantly increased and MLC-2f and MLC-3f somewhat reduced. Fast initial active shortening seems almost unaffected in mdx muscle.