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Kinetics of Multiplex Hybridization: Mechanisms and Implications

Electrical and Computer Engineering, University of Utah, Salt Lake City, Utah

Correspondence: Address reprint requests to S. Blair, E-mail: blair{at}ece.utah.edu.

Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous interpretation of experimental data. We suggest here that a simplified three-component kinetic model may present a sufficient approximation to describe the general case of DNA sensing in a complex sample milieu. We show that, by analyzing the real-time hybridization kinetics of a nontarget species, we can perform quantitative analysis of unlabeled targets of interest within a broad dynamic range of concentrations.