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Biophys. J. BioFAST: First Published October 29, 2004. doi:10.1529/biophysj.104.049866
© 2004 by the Biophysical Society.

A more recent version of this article appeared on January 1, 2005.
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ELECTROPHYSIOLOGY

The Interpretation of Current Clamp Recordings in the Cell-Attached Patch Clamp Configuration

Michael J. Mason 1*, Anna K. Simpson 1, Martyn P. Mahaut-Smith 1 and Hugh P.C. Robinson 1

1 University of Cambridge

* To whom correspondence should be addressed. E-mail: mjm39{at}cam.ac.uk.

Submitted on July 16, 2004
Revised on August 26, 2004
Accepted on 19 October 2004


  Abstract
In the present experiments we have investigated the feasibility and accuracy of recording steady-state and dynamic changes in transmembrane potential non-invasively across an intact cell-attached patch using the current clamp mode of a conventional patch clamp amplifier. Using an equivalent circuit mimicking simultaneous whole-cell voltage clamp and cell-attached current clamp recordings we have defined both mathematically and experimentally the relationship between the membrane patch resistance, the seal resistance and the fraction of the whole-cell potential recorded across an intact membrane patch. This analysis revealed a steep increase in the accuracy of the recording of steady-state membrane potential as the seal to membrane ratio increases from 0 and approaches 100% accuracy as the ratio approaches infinity. Membrane potential measurements across intact cell-attached patches in rat basophilic leukemia cells and rat megakaryocytes revealed a surprisingly high degree of accuracy and demonstrated the ability of this non-invasive technique to follow dynamic changes in potential in non-excitable cells.

Key Words: RBL cell, cell-attached, current clamp, megakaryocyte, membrane potential, non-invasive




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Copyright © 2004 by the Biophysical Society.