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Biophys. J. BioFAST: First Published April 29, 2005. doi:10.1529/biophysj.104.050401
© 2005 by the Biophysical Society.

A more recent version of this article appeared on July 1, 2005.
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Trapping, deformation and rotation of giant unilamellar vesicles in octode dielectrophoretic field cages

Jonas Korlach 1, Christoph Reichle 2, Torsten Müller 3, Thomas Schnelle 3* and Watt W Webb 4

1 Nanofluidics Inc.
2 Humboldt-Universität zu Berlin
3 Evotec Technologies GmbH
4 Cornell University

* To whom correspondence should be addressed. E-mail: thomas.schnelle{at}evotec-technologies.com.

Submitted on July 26, 2004
Revised on August 25, 2004
Accepted on 28 March 2005


  Abstract
The behavior of free-standing lipid bilayer membranes under the influence of dielectric force potentials was studied by trapping, holding and rotating individual giant unilamellar vesicles (GUVs) inside dielectrophoretic microfield cages. Using laser scanning confocal microscopy and three-dimensional image reconstructions of GUVs labeled with fluorescent membrane probes, field strength and frequency dependent vesicle deformations were observed which are explained by calculations of the dielectric force potentials inside the cage. Dynamical membrane properties under the influence of the field cage were studied by fluorescence correlation spectroscopy (FCS), circumventing potential artifacts associated with measurements involving GUV immobilization on support surfaces. Lipid transport could be accelerated markedly by the applied fields, aided by hydrodynamic fluid streaming which was also studied by FCS.

Key Words: Dielectrophoresis, Fluorescence Correlation Spectroscopy, Giant Unilamellar Vesicles, lipid bilayer membranes, octode field cages




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K. A. Riske and R. Dimova
Electric Pulses Induce Cylindrical Deformations on Giant Vesicles in Salt Solutions
Biophys. J., September 1, 2006; 91(5): 1778 - 1786.
[Abstract] [Full Text] [PDF]


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Copyright © 2005 by the Biophysical Society.