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Biophys. J. BioFAST: First Published January 14, 2005. doi:10.1529/biophysj.104.050823
© 2005 by the Biophysical Society.

A more recent version of this article appeared on April 1, 2005.
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Multilayer structures in lipid monolayer films containing surfactant protein C: Effects of Cholesterol and POPE

Stefan Malcharek 1, Andreas Hinz 1, Lutz Hilterhaus 1 and Hans-Joachim Galla 1*

1 Institut fuer Biochemie, Westfaelische Wilhelms-Universitaet Muenster

* To whom correspondence should be addressed. E-mail: gallah{at}uni-muenster.de.

Submitted on August 2, 2004
Revised on September 25, 2004
Accepted on 3 January 2005


  Abstract
The influence of cholesterol and POPE on lung surfactant model systems consisting of DPPC/DPPG (80:20) and DPPC/DPPG/SP-C (80:20:0.4) has been investigated. Cholesterol leads to a higher condensation of the monolayers, whereas the isotherms of model lung surfactant films containing POPE show a higher compressibility at low surface pressures. An increasing amount of liquid expanded domains can be visualized by means of fluorescence light microscopy in lung surfactant monolayers after addition of cholesterol or POPE. At high surface pressures protrusions are formed, which differ in size and shape as a function of the content of cholesterol or POPE. Low amounts of cholesterol (10 mol%) lead to an increasing number of protrusions, which also grow in size. This is interpreted as a stabilizing effect of cholesterol on bilayers formed underneath the monolayer. High amounts of cholesterol (30 mol%) cause an increased monolayer rigidity and prevent multilayer formation. In contrast, POPE as a non-bilayer lipid stabilizes the edges of protrusions and facilitates their formation, leading to more narrow protrusions. The shape of protrusions is thereby more influenced than their height.

Key Words: SP-C, fluorescence light microscopy, lung surfactant, protrusion, scanning force microscopy




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Copyright © 2005 by the Biophysical Society.