help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published January 14, 2005. doi:10.1529/biophysj.104.051706
© 2005 by the Biophysical Society.

A more recent version of this article appeared on March 1, 2005.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.104.051706v1
88/3/2299    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Heinrich, V.
Right arrow Articles by Evans, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Heinrich, V.
Right arrow Articles by Evans, E.

CELL BIOPHYSICS

Nano-to-Micro Scale Dynamics of P-selectin Detachment from Leukocyte Interfaces: II. Tether flow terminated by P-selectin dissociation from PSGL-1

Volkmar Heinrich 1, Andrew Leung 2 and Evan Evans 1*

1 Boston University
2 University of British Columbia

* To whom correspondence should be addressed. E-mail: evans{at}physics.ubc.ca.

Submitted on August 23, 2004
Revised on October 21, 2004
Accepted on 20 December 2004


  Abstract
We have used a biomembrane force probe (BFP) decorated with P-selectin to form point attachments with PSGL-1 receptors on a human neutrophil (PMN) in a calcium-containing medium and then to quantify the forces experienced by the attachment during retraction of the PMN at fixed speed. From first touch to final detachment, the typical force history exhibited the following sequence of events: (i) an initial linear-elastic displacement of the PMN surface, (ii) an abrupt crossover to viscoplastic flow that signaled membrane separation from the interior cytoskeleton and the beginning of a membrane tether, and (iii) the final detachment from the probe tip most often by one precipitous step of P-selectin:PSGL-1 dissociation. Analyzing the initial elastic response and membrane unbinding from the cytoskeleton in our companion paper (Evans et al., 2004a), we focus in this paper on the regime of tether extrusion that nearly always occurred before release of the extracellular adhesion bond at pulling speeds ≥ 1 µm/s. The force during tether growth appeared to approach a plateau at long times. Examined over a large range of pulling speeds up to 150 µm/s, the plateau force exhibited a significant shear-thinning as indicated by a weak power law dependence on pulling speed, ƒ {infty} = 60pN({nu}pull/µm/s)0.25. Using this shear thinning response to describe the viscous element in a nonlinear Maxwell-like fluid model, we show that a weak serial-elastic component with a stiffness of ~ 0.07 pN/nm provides good agreement with the time course of the tether force approach to the plateau under constant pulling speed.

Key Words: P-selectin detachment from leukocyte receptors, rheology of tether extrusion from leukocytes




This article has been cited by other articles:


Home page
 Biophys. JHome page
J. Schmitz, M. Benoit, and K.-E. Gottschalk
The Viscoelasticity of Membrane Tethers and Its Importance for Cell Adhesion
Biophys. J., August 1, 2008; 95(3): 1448 - 1459.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
G. Girdhar and J.-Y. Shao
Simultaneous Tether Extraction from Endothelial Cells and Leukocytes: Observation, Mechanics, and Significance
Biophys. J., December 1, 2007; 93(11): 4041 - 4052.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. Jauffred, T. H. Callisen, and L. B. Oddershede
Visco-Elastic Membrane Tethers Extracted from Escherichia coli by Optical Tweezers
Biophys. J., December 1, 2007; 93(11): 4068 - 4075.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
B. Liu, C. J. Goergen, and J.-Y. Shao
Effect of Temperature on Tether Extraction, Surface Protrusion, and Cortical Tension of Human Neutrophils
Biophys. J., October 15, 2007; 93(8): 2923 - 2933.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
N. Borghi and F. Brochard-Wyart
Tether Extrusion from Red Blood Cells: Integral Proteins Unbinding from Cytoskeleton
Biophys. J., August 15, 2007; 93(4): 1369 - 1379.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. Heinrich and C. Ounkomol
Force versus Axial Deflection of Pipette-Aspirated Closed Membranes
Biophys. J., July 15, 2007; 93(2): 363 - 372.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
F. Brochard-Wyart, N. Borghi, D. Cuvelier, and P. Nassoy
Hydrodynamic narrowing of tubes extruded from cells
PNAS, May 16, 2006; 103(20): 7660 - 7663.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A.-S. Smith, B. G. Lorz, U. Seifert, and E. Sackmann
Antagonist-Induced Deadhesion of Specifically Adhered Vesicles
Biophys. J., February 1, 2006; 90(3): 1064 - 1080.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
K. E. Edmondson, W. S. Denney, and S. L. Diamond
Neutrophil-Bead Collision Assay: Pharmacologically Induced Changes in Membrane Mechanics Regulate the PSGL-1/P-Selectin Adhesion Lifetime
Biophys. J., November 1, 2005; 89(5): 3603 - 3614.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
E. Evans, V. Heinrich, A. Leung, and K. Kinoshita
Nano- to Microscale Dynamics of P-Selectin Detachment from Leukocyte Interfaces. I. Membrane Separation from the Cytoskeleton
Biophys. J., March 1, 2005; 88(3): 2288 - 2298.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by the Biophysical Society.