Interaction between Lipid Monolayers and Poloxamer 188: An X-ray Reflectivity and Diffraction Study

¹ the University of Chicago
² Los Alamos National Laboratory
³ Ris?National Laboratory

^* To whom correspondence should be addressed. E-mail: kayeelee{at}uchicago.edu.

Submitted on September 3, 2004
Revised on November 3, 2004
Accepted on 27 June 2005

	Abstract

The mechanism by which poloxamer 188 (P188) seals a damaged cell membrane is examined using the lipid monolayer as a model system. X-ray reflectivity and grazing-incidence x-ray diffraction results show that at low nominal lipid density, P188, by physically occupying the available area and phase separating from the lipids, forces the lipid molecules to pack tightly and restore the barrier function of the membrane. Upon compression to bilayer equivalent pressure, P188 is squeezed out from the lipid monolayer, allowing a graceful exit of P188 when the membrane integrity is restored.

Key Words: corral, electropermeabilized cell membrane, grazing incidence x-ray diffraction, lipid monolayer, poloxamer, x-ray reflectivity

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