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Biophys. J. BioFAST: First Published June 10, 2005. doi:10.1529/biophysj.104.054510
© 2005 by the Biophysical Society.

A more recent version of this article appeared on September 1, 2005.
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MEMBRANES

Effect of Salicylate on the Elasticity, Bending Stiffness, and Strength of SOPC Membranes

Yong Zhou 1 and Robert M. Raphael 1*

1 Rice University

* To whom correspondence should be addressed. E-mail: rraphael{at}rice.edu.

Submitted on November 18, 2004
Revised on February 1, 2005
Accepted on 6 June 2005


  Abstract
Salicylate is a small amphiphilic molecule which has diverse effects on membranes and membrane-mediated processes. We have utilized micropipette aspiration of giant unilamellar vesicles to determine salicylate's effects on lecithin membrane elasticity, bending rigidity and strength. Salicylate effectively reduces the apparent area compressibility modulus and bending modulus of membranes in a dose-dependent manner at concentrations above 1 mM, but does not greatly alter the actual elastic compressibility modulus at the maximal tested concentration of 10 mM. The effect of salicylate on membrane strength was investigated using dynamic tension spectroscopy (DTS), which revealed that salicylate increases the frequency of spontaneous defect formation and lowers the energy barrier for hole formation. The mechanical and dynamic tension experiments are consistent and support a picture in which salicylate disrupts membrane stability by decreasing membrane stiffness and membrane thickness. The tension-dependent partitioning of salicylate was utilized to calculate the molecular volume of salicylate in the membrane. The free energy of transfer for salicylate insertion into the membrane and the corresponding partition coefficient were also estimated, and indicated favorable salicylate-membrane interactions. The mechanical changes induced by salicylate may affect several biological processes, especially those associated with membrane curvature and permeability.

Key Words: SOPC, bending stiffness, compressibility, dynamic tension, micropipette aspiration, salicylate




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Copyright © 2005 by the Biophysical Society.