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Biophys. J. BioFAST: First Published December 30, 2004. doi:10.1529/biophysj.104.055061
© 2004 by the Biophysical Society.

A more recent version of this article appeared on March 1, 2005.
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MEMBRANES

Reinvestigtion by phosphorus NMR of lipid distribution in bicelles

Mohamed N TRIBA 1, Dror E Warschawski 1 and Philippe F DEVAUX 1*

1 Institut de Biologie Physico-Chimique

* To whom correspondence should be addressed. E-mail: philippe.devaux{at}ibpc.fr.

Submitted on October 26, 2004
Revised on November 28, 2004
Accepted on 22 December 2004


  Abstract
Mixtures of dimyristoyl-phosphatidylcholine (DMPC), and dihexanoyl-phosphatidylcholine (DHPC) in water form disks also called bicelles and different bilayer organisations when the mol ratio of the two lipids and the temperature are varied. The spontaneous orientation in a magnetic field of these bilayers above the transition temperature Tm of DMPC is an attractive property that was successfully used to investigate protein structure by NMR. In this article, we have attempted to give an overview of all structural transformations of DMPC/DHPC mixtures that can be inferred from broad band 31P-NMR spectroscopy between 5 and 60°C. We show that above a critical temperature, Tv, perforated vesicles progressively replace alignable structures. The holes in these vesicles disappear above a new temperature threshold, Th. The driving force for these temperature dependent transformations that has been overlooked in previous studies is the increase of DHPC miscibility in the bilayer domain above Tm. Accordingly, we propose a new model (the mixed bicelle model) that emphasizes that consequence of the mixing. This investigation shows that the various structures of DMPC in the presence of increasing mol ratios of the short chain DHPC is reminiscent of the observation put forward by several laboratories investigating solubilization and reconstitution of biological membranes

Key Words: dihexanoyl phosphatidylcholine, dimyristoyl phosphatidylcholine, lipid miscibility, oriented bilayers, perforated vesicles, solid state NMR




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Copyright © 2004 by the Biophysical Society.