help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published July 29, 2005. doi:10.1529/biophysj.104.057026
© 2005 by the Biophysical Society.

A more recent version of this article appeared on October 1, 2005.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.104.057026v1
89/4/2888    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Trache, A.
Right arrow Articles by Meininger, G. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trache, A.
Right arrow Articles by Meininger, G. A.

OTHER

Histamine effects on endothelial cell fibronectin interaction studied by atomic force microscopy

Andreea Trache 1, Jerome P. Trzeciakowski 1, Lesley A. Gardiner 1, Zhe Sun 1, Mariappan Muthuchamy 1, Mingzhang Guo 1, Sarah Y. Yuan 1 and Gerald A. Meininger 1*

1 The Texas A&M University System Health Science Center

* To whom correspondence should be addressed. E-mail: gam{at}tamu.edu.

Submitted on December 1, 2004
Revised on February 22, 2005
Accepted on 7 July 2005


  Abstract
Atomic Force Microscopy (AFM) was used to investigate the cellular response to histamine, one of the major inflammatory mediators that cause endothelial hyperpermeability and vascular leakage. AFM probes were labeled with fibronectin and used to measure binding strength between {alpha}5{beta}1 integrin and fibronectin by quantifying the force required to break single fibronectin-integrin bonds. The cytoskeletal changes, binding probability and adhesion force before and after histamine treatment on endothelial cells were monitored. Cell topography measurements indicated that histamine induced cell shrinkage. Local cell stiffness and binding probability increased two fold after histamine treatment. The force necessary to rupture single {alpha}5{beta}1-fibronectin bond increased from 34.0 ± 0.5 pN in control cells to 39 ± 1 pN after histamine treatment. Experiments were also conducted to confirm the specificity of the {alpha}5{beta}1- fibronectin interaction. In the presence of soluble GRGDdSP the probability of adhesion events decreased more than 50% while the adhesion force between {alpha}5{beta}1 and fibronectin remained unchanged. These data indicate that extracellular matrix-integrin interactions play an important role in the endothelial cell response to changes of external chemical mediators. These changes can be recorded as direct measurements on live endothelial cells by using atomic force microscopy.

Key Words: cell adhesion, cell stiffness, extracellular matrix proteins, integrins




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
J. Helenius, C.-P. Heisenberg, H. E. Gaub, and D. J. Muller
Single-cell force spectroscopy
J. Cell Sci., June 1, 2008; 121(11): 1785 - 1791.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Yersin, H. Hirling, S. Kasas, C. Roduit, K. Kulangara, G. Dietler, F. Lafont, S. Catsicas, and P. Steiner
Elastic Properties of the Cell Surface and Trafficking of Single AMPA Receptors in Living Hippocampal Neurons
Biophys. J., June 15, 2007; 92(12): 4482 - 4489.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. H. Wu
Endothelial focal adhesions and barrier function
J. Physiol., December 1, 2005; 569(2): 359 - 366.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by the Biophysical Society.