help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published March 25, 2005. doi:10.1529/biophysj.105.061788
© 2005 by the Biophysical Society.

A more recent version of this article appeared on May 1, 2005.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow All Versions of this Article:
biophysj.105.061788v1
88/5/L33    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Digman, M. A.
Right arrow Articles by Gratton, E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Digman, M. A.
Right arrow Articles by Gratton, E.

BIOPHYSICAL LETTERS

Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure

Michelle A. Digman 1, Parijat Sengupta 1, Paul W. Wiseman 2, Claire M. Brown 3, Alan R. Horwitz 3 and Enrico Gratton 1*

1 University of Illinois at Urbana-Champaign
2 McGill University
3 University of Virginia

* To whom correspondence should be addressed. E-mail: egratton22{at}yahoo.com.

Submitted on February 22, 2005
Revised on March 14, 2005
Accepted on 21 March 2005


  Abstract
Images obtained with a laser scanning microscope contain a time structure that can be exploited to measure fast dynamics of molecules in solution and in cells. The spatial correlation approach provides a simple algorithm to extract this information. We describe the analysis used to process laser scanning images of solutions and cells to obtain molecular diffusion constant in the microsecond to second time scale.

Key Words: correlation spectroscopy, fluorescence, image correlation, laser confocal microscopy




This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
V. Vukojevic, M. Heidkamp, Y. Ming, B. Johansson, L. Terenius, and R. Rigler
Quantitative single-molecule imaging by confocal laser scanning microscopy
PNAS, November 25, 2008; 105(47): 18176 - 18181.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
M. Vendelin and R. Birkedal
Anisotropic diffusion of fluorescently labeled ATP in rat cardiomyocytes determined by raster image correlation spectroscopy
Am J Physiol Cell Physiol, November 1, 2008; 295(5): C1302 - C1315.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. A. Digman, C. M. Brown, A. R. Horwitz, W. W. Mantulin, and E. Gratton
Paxillin Dynamics Measured during Adhesion Assembly and Disassembly by Correlation Spectroscopy
Biophys. J., April 1, 2008; 94(7): 2819 - 2831.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
A. Celli, S. Beretta, and E. Gratton
Phase Fluctuations on the Micron-Submicron Scale in GUVs Composed of a Binary Lipid Mixture
Biophys. J., January 1, 2008; 94(1): 104 - 116.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. J. Saxton
A Biological Interpretation of Transient Anomalous Subdiffusion. I. Qualitative Model
Biophys. J., February 15, 2007; 92(4): 1178 - 1191.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y.-l. Wang, K. M. Hahn, R. F. Murphy, and A. F. Horwitz
From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006
J. Cell Biol., August 14, 2006; 174(4): 481 - 484.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
V. K. Ramanujan, G. Biener, and B. A. Herman
Scaling Behavior in Mitochondrial Redox Fluctuations
Biophys. J., May 15, 2006; 90(10): L70 - L72.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
D. L. Kolin, S. Costantino, and P. W. Wiseman
Sampling Effects, Noise, and Photobleaching in Temporal Image Correlation Spectroscopy
Biophys. J., January 15, 2006; 90(2): 628 - 639.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
M. A. Digman, C. M. Brown, P. Sengupta, P. W. Wiseman, A. R. Horwitz, and E. Gratton
Measuring Fast Dynamics in Solutions and Cells with a Laser Scanning Microscope
Biophys. J., August 1, 2005; 89(2): 1317 - 1327.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2005 by the Biophysical Society.