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Biophys. J. BioFAST: First Published April 22, 2005. doi:10.1529/biophysj.105.061994
© 2005 by the Biophysical Society.

A more recent version of this article appeared on July 1, 2005.
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Glioma Expansion in Collagen I Matrices: Analyzing Collagen Concentration-Dependent Growth and Motility Patterns

Laura J Kaufman 1*, Clifford P. Brangwynne 2, Karen E. Kasza 2, Emma Filippidi 2, Vernita D. Gordon 3, Thomas S. Deisboeck 4 and David A. Weitz 2

1 Columbia University
2 Harvard University
3 University of Edinburgh
4 Massachusetts General Hospital; Harvard Medical School

* To whom correspondence should be addressed. E-mail: kaufman{at}chem.columbia.edu.

Submitted on February 25, 2005
Revised on March 25, 2005
Accepted on 21 April 2005


  Abstract
We study the growth and invasion of glioblastoma multiforme (GBM) in three-dimensional (3D) collagen I matrices of varying collagen concentration. Phase contrast microscopy studies of the entire GBM system show that invasiveness at early times is limited by available collagen fibers. At early times, high collagen concentration correlates with more effective invasion. Conversely, high collagen concentration correlates with inhibition in the growth of the central portion of GBM, the multicellular tumor spheroid (MTS). Analysis of confocal reflectance images of the collagen matrices quantifies how the collagen matrices differ as a function of concentration. Studying invasion on the length scale of individual invading cells with a combination of confocal and coherent anti-Stokes Raman Scattering (CARS) microscopy reveals that the invasive GBM cells rely heavily on cell-matrix interactions during invasion and remodeling.

Key Words: CARS microscopy, cell migration, collagen matrix, glioblastoma multiforme




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Copyright © 2005 by the Biophysical Society.