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Biophys. J. BioFAST: First Published May 6, 2005. doi:10.1529/biophysj.105.064816
© 2005 by the Biophysical Society.

A more recent version of this article appeared on July 1, 2005.
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BIOPHYSICAL LETTERS

Cholesterol Enriched Lipid Domains can be Visualized by di-4-ANEPPDHQ with Linear and Non-Linear Optics

Lei Jin 1, Andrew C Millard 1, Joseph P Wuskell 1, Heather A Clark 1 and Leslie M Loew 1*

1 UConn Health Center

* To whom correspondence should be addressed. E-mail: les{at}volt.uchc.edu.

Submitted on April 19, 2005
Revised on April 25, 2005
Accepted on 4 May 2005


  Abstract
We present a membrane-staining dye, di-4-ANEPPDHQ, which differentiates liquid-ordered phases from -disordered phases coexisting in model membranes under both linear and non-linear microscopies. The dye's fluorescence emission spectrum is blue-shifted 60nm in liquid-ordered phases compared with -disordered phases, and shows strong second harmonic generation in the liquid-disordered phase compared with the -ordered phase. The ease of staining and the ability of this single dye to detect both phases should lead to broad applications in biophysical studies of lipid domains in model membranes and cells.

Key Words: 2-photon, fluorescence, lipid domain, membrane, rafts, second harmonic generation




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Copyright © 2005 by the Biophysical Society.