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Biophys. J. BioFAST: First Published May 19, 2006. doi:10.1529/biophysj.105.067561
© 2006 by the Biophysical Society.

A more recent version of this article appeared on August 1, 2006.
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PHOTOBIOPHYSICS

Reconstruction of a kinetic model of the chromatophore vesicles from Rhodobacter sphaeroides

Tihamer Geyer 1* and Volkhard Helms 1

1 Saarland University

* To whom correspondence should be addressed. E-mail: tihamer.geyer{at}bioinformatik.uni-saarland.de.

Submitted on May 30, 2005
Revised on August 29, 2005
Accepted on 26 April 2006


  Abstract
We present a molecular model of a chromatophore vesicle from Rhodobacter sphaeroides. These vesicles are ideal benchmark systems for molecular and systemic simulations, because they have been well studied, they are small, and they are naturally separated from their cellular environment. To set up a photosynthetic chain working under steady state conditions, we compiled from the experimental literature the specific activities and geometries that have been determined for their constituents. This data then allowed defining the stoichiometries for all membrane proteins. This paper contains the kinetic part of the reconstructed model, while the spatial reconstruction is presented in [Geyer, Helms, Biophys. J.]. By considering the transport properties of the cytochrome c2 and ubiquinone pools, we show that their size and oxidation states allow for an efficient buffering of the statistical fluctuations that arise from the small size of the vesicles. Stoichiometric and kinetic considerations indicate that a typical chromatophore vesicle of Rb. sphaeroides with a diameter of 45 nm should contain about five <bc1 monomers.

Key Words: Rhodobacter sphaeroides, chromatophore vesicle, conversion chain, cytochrome bc1 complex, purple bacteria, systems biology




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Copyright © 2006 by the Biophysical Society.