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Biophys. J. BioFAST: First Published September 8, 2005. doi:10.1529/biophysj.105.068080
© 2005 by the Biophysical Society.

A more recent version of this article appeared on December 1, 2005.
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MEMBRANES

MANY-BODY EFFECT OF ANTIMICROBIAL PEPTIDES: On the correlation between lipid's spontaneous curvature and pore formation

Ming-Tao Lee 1, Wei-Chin Hung 2, Fang-Yu Chen 1 and Huey W. Huang 3*

1 National Central University
2 Chinese Military Academy
3 Rice University

* To whom correspondence should be addressed. E-mail: hwhuang{at}rice.edu.

Submitted on June 7, 2005
Revised on July 20, 2005
Accepted on 24 August 2005


  Abstract
Recently we have shown that the free energy for pore formation induced by antimicrobial peptides contains a term representing peptide-peptide interactions mediated by membrane thinning. This many-body effect gives rise to the cooperative concentration dependence of peptides' activities. Here we performed oriented circular dichroism and x-ray diffraction experiments to study the lipid dependence of this many-body effect. In particular we studied the correlation between lipid's spontaneous curvature and peptide's threshold concentration for pore formation, by adding PE and lysoPC to PC bilayers. Previously this correlation exhibited by magainin and melittin was argued to support the toroidal model for the pores. Here we found similar correlations exhibited by melittin and alamethicin. We found that the main effect of varying the spontaneous curvature of lipid is changing the degree of membrane thinning, which in turns influences the threshold concentration for pore formation. We discuss how to understand the lipid dependence of membrane thinning.

Key Words: barrel-stave model, lamellar x-ray diffraction, lethal concentration, membrane-peptide interactions, oriented circular dichroism, toroidal model




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Copyright © 2005 by the Biophysical Society.