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Biophys. J. BioFAST: First Published January 13, 2006. doi:10.1529/biophysj.105.068205
© 2006 by the Biophysical Society.

A more recent version of this article appeared on April 1, 2006.
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CELL BIOPHYSICS

Probing surface structures of Shewanella spp. by microelectrophoresis

Etienne Dague 1, Jérôme Duval 2, Frédéric Jorand 3, Fabien Thomas 4 and Fabien Gaboriaud 5*

1 Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, UMR 7564, CNRS, UHP Nancy I
2 Department of Physical Chemistry and Colloid Science, Wageningen University
3 Laboratoire de Chimie Physique et Microbiologie pour l'Environnement, UMR 7564, CNRS, IUHP
4 Laboratoire Environnement et Minéralurgie, UMR 7569 CNRS-INPL
5 LCPME (CNRS/UHP)

* To whom correspondence should be addressed. E-mail: gaboriaud{at}lcpme.cnrs-nancy.fr.

Submitted on June 8, 2005
Revised on November 16, 2005
Accepted on 14 December 2005


  Abstract
Long-range electrostatic forces influence substantially bacterial interactions and bacterial adhesion during the preliminary steps of biofilm formation. The strength of these forces depends strongly on the structure of the bacterium surfaces investigated. The latter may be addressed from appropriate analysis of electrophoretic mobility measurements. Due to the permeable character of the bacterium wall and/or surrounding polymeric layer, bacteria may be regarded as paradigms of soft bioparticles. The electrophoretic motion of such particles in a DC electric field differs considerably from that of their rigid counterparts in the sense that electroosmotic flow takes place around and within the soft surface layer. Recent developments of electrokinetic theories for soft particles now render possible the evaluation of the softness degree (or equivalently the hydrodynamic permeability) from the raw electrokinetic data. In this paper, the electrophoretic mobilities of three Shewanella strains (MR-4, CN32, and BrY)presenting various and well characterized phenotypes of polymeric fringe are reported over a wide range of pH and ionic strength conditions. The data are quantitatively analysed on the basis of a rigorous numerical evaluation of the governing electrostatic and hydrodynamic equations for soft particles. It is clearly shown how the peculiar surface structures of the bacteria investigated are reflected in their electro-hydrodynamic properties.

Key Words: Electrophoretic mobility, Heterogeneity, Shewanella, Soft particle electrophoresis theory




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J. Langlet, F. Gaboriaud, C. Gantzer, and J. F. L. Duval
Impact of Chemical and Structural Anisotropy on the Electrophoretic Mobility of Spherical Soft Multilayer Particles: The Case of Bacteriophage MS2
Biophys. J., April 15, 2008; 94(8): 3293 - 3312.
[Abstract] [Full Text] [PDF]


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Copyright © 2006 by the Biophysical Society.