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Biophys. J. BioFAST: First Published January 13, 2006. doi:10.1529/biophysj.105.071530
© 2006 by the Biophysical Society.

A more recent version of this article appeared on April 1, 2006.
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Oligomerization and Aggregation of Bovine Pancreatic Ribonuclease A: Characteristic Events Observed by FTIR Spectroscopy

Yong-Bin Yan 1*, Jun Zhang 1, Hua-Wei He 1 and Hai-Meng Zhou 1

1 Tsinghua University

* To whom correspondence should be addressed. E-mail: ybyan{at}tsinghua.edu.cn.

Submitted on July 27, 2005
Revised on September 13, 2005
Accepted on 24 October 2005


  Abstract
Nonnative protein aggregation, which is a common feature in biotechnology, is also a clinical feature in more than 20 serious degenerative diseases. We studied the specific events of bovine pancreatic ribonuclease A thermal aggregation by a combination of second derivative infrared analysis and two-dimensional infrared correlation spectroscopy. By comparing the events that occur in reversible and irreversible thermal unfolding processes, certain events that were related to protein aggregation were characterized. Particularly, a band that appeared at high temperatures was assigned to the cross {beta}-structures in oligomers. The effect of pH, NaCl and ethanol on ribonuclease A oligomerization as well as further aggregation induced by heat were studied and dissimilar effects of these additions were found. Basic pH and NaCl could accelerate the thermal aggregation but did not affect the formation of oligomers, while ethanol could increase both the aggregation rate and the population of oligomers. Our results suggested that the aggregation of RNase A might be initiated by hydrophobic interactions, controlled by oligomerization and mediated by electrostatic interactions. Moreover, the strategy of using second derivative IR and 2D IR analysis might provide a potential powerful tool to study the events that are directly related to the initiation of protein aggregation.

Key Words: infrared spectroscopy, protein aggregation, protein oligomerization, second derivative, two-dimensional correlation spectroscopy




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Copyright © 2006 by the Biophysical Society.