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Biophys. J. BioFAST: First Published February 3, 2006. doi:10.1529/biophysj.105.072959
© 2006 by the Biophysical Society.

A more recent version of this article appeared on May 1, 2006.
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MEMBRANES

Phase behavior of lipid monolayers containing DPPC and cholesterol analogs

Sarah L. Keller 1* and Benjamin L. Stottrup 2

1 University of Washington
2 Augsburg College

* To whom correspondence should be addressed. E-mail: slkeller{at}chem.washington.edu.

Submitted on August 19, 2005
Revised on September 9, 2005
Accepted on 5 January 2006


  Abstract
We investigate the miscibility phase behavior of lipid monolayers containing a wide variety of sterols. Six of the sterols satisfy a definition of "membrane active sterols" in bilayers (cholesterol, epicholesterol, lathosterol, dihydrocholesterol, ergosterol, and desmosterol), and six do not (25-hydroxycholesterol, lanosterol, androstenolone, coprostanol, cholestane, and cholestenone) [Y. Barenholz, 2002, Prog. Lipid Res. 41, 1-5]. We find that monolayers containing dipalmitoyl phosphatidylcholine (DPPC) mixed with membrane active sterols generally produce phase diagrams containing two distinct regions of immiscible liquid phases, whereas those with membrane inactive sterols generally do not. This observation establishes a correlation between lipid monolayers and bilayers. It also demonstrates that the ability to form two regions of immiscibility in monolayers is not one of the biophysical attributes that explains cholesterol's predominance in animal cell membranes. Furthermore, we find unusual phase behavior for DPPC monolayers containing 25-hydroxycholesterol, which produce both an upper and a lower miscibility transition. The lower transition correlates with a sharp change of slope in the pressure-area isotherm.

Key Words: ergosterol, lanosterol, liquid, miscibility, phase diagram, sterol




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Copyright © 2006 by the Biophysical Society.