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Biophys. J. BioFAST: First Published October 14, 2005. doi:10.1529/biophysj.105.073866
© 2005 by the Biophysical Society.

A more recent version of this article appeared on December 1, 2005.
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Dusan Chorvat Jr
Jana Kirchnerova
Michal Cagalinec
Jozef Smolka
Anton Mateasik
Alzbeta Chorvatova
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Spectral unmixing of flavin autofluorescence components in cardiac myocytes

Dusan Chorvat Jr 1*, Jana Kirchnerova 2, Michal Cagalinec 2, Jozef Smolka 1, Anton Mateasik 1 and Alzbeta Chorvatova 3

1 International Laser Centre, Bratislava
2 Faculty of Mathematics, Physics and Informatics, Comenius University, Bratislava
3 Research Centre of St.Justine Hospital, Montreal

* To whom correspondence should be addressed. E-mail: dusan{at}ilc.sk.

Submitted on September 2, 2005
Revised on September 21, 2005
Accepted on 28 September 2005


  Abstract
We applied linear unmixing approach to reveal individual components of intrinsic flavin fluorescence signal recorded in living cardiac cells by spectrally-resolved confocal microscopy. Responses of whole-cell autofluorescence to modulators of cell metabolism and respiration were used as a tool of separation of its components; their spectral profiles, estimated by principal component analysis, correspond to free FAD and FAD bound to different enzymes of electron transport chain.

Key Words: cardiac myocytes, flavin autofluorescence, linear unmixing, metabolic modulation, spectral imaging




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Copyright © 2005 by the Biophysical Society.