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Biophys. J. BioFAST: First Published January 20, 2006. doi:10.1529/biophysj.105.074252
© 2006 by the Biophysical Society.

A more recent version of this article appeared on April 1, 2006.
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Dolores C. Carrer
Bruno Maggio
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MEMBRANES

Effects of a short chain ceramide on bilayer domain formation, thickness and chain mobility

Dolores C. Carrer 1*, Shirley Schreier 2, Martin Patrito 3 and Bruno Maggio 4

1 SDU, Memphys
2 Instituto de Quimica, USP
3 Dpto. Fisico Quimica, INFICQ, Fac. Cs. Quimicas, UNC
4 Facultad de Ciencias Quimicas

* To whom correspondence should be addressed. E-mail: dcarrer{at}gmail.com.

Submitted on September 14, 2005
Revised on October 18, 2005
Accepted on 12 December 2005


  Abstract
An important part of natural ceramides contain asymmetric hydrocarbon chains. We have used calorimetry, AFM and EPR to study the effect of ceramide chain asymmetry in mixtures of C8Cer with DMPC as a model system of hydrocarbon chain disparity. A phase diagram is provided along with information on the thickness of the membrane and the mobility of the chains at different temperatures both below and above the phase transition temperature of the mixtures. The results indicate a partial interdigitation of C8Cer chains in the gel phase, producing a correlation between the organization of both hemilayers. Our data suggest that the effects of ceramides on biomembranes may be bimodal and similar to those of cholesterol.

Key Words: AFM, C8Cer, EPR, calorimetry, interdigitation, phase diagram




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Copyright © 2006 by the Biophysical Society.