Sonoporation from jetting cavitation bubbles

¹ University of Twente
² University of Twente and Erasmus University
³ University of Munich

^* To whom correspondence should be addressed. E-mail: c.d.ohl{at}tnw.utwente.nl.

Submitted on September 30, 2005
Revised on January 12, 2006
Accepted on 18 August 2006

	Abstract

The fluid dynamic interaction of cavitation bubbles with adherent cells on a substrate is investigated experimentally and theoretically. We find that the non-spherical collapse of bubbles near to the boundary is responsible for cell detachment. High-speed photography reveals that a wall bounded flow leads to the detachment of cells. Cells at the edge of the circular area of detachment are found to be permanently porated; whereas cells at some distance from the detachment area undergo viable cell membrane poration (sonoporation). The wall flow field leading to cell detachment is modeled with a self-similar solution for a wall jet, together with a kinetic ansatz of adhesive bond rupture. The self-similar solution for the -type wall jet compares very well with the full solution of the Navier-Stokes equation for a jet of finite thickness. Apart from annular sites of sonoporation we also find more homogenous patterns of molecule delivery with no cell detachment.

Key Words: HeLa cells, boundary layer flow, cell detachment, high-speed photography, wall jet

This article has been cited by other articles:

				Y. Zhou, J. Cui, and C. X. Deng Dynamics of Sonoporation Correlated with Acoustic Cavitation Activities Biophys. J., April 1, 2008; 94(7): L51 - L53. [Abstract] [Full Text] [PDF]