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Biophys. J. BioFAST: First Published November 11, 2005. doi:10.1529/biophysj.105.075556
© 2005 by the Biophysical Society.

A more recent version of this article appeared on January 1, 2006.
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BIOPHYSICAL LETTERS

Melanosomes Transported by Myosin V in Xenopus Melanophores Perform Slow 35 nm Steps

Valeria Levi 1, Vladimir I Gelfand 2, Anna S Serpinskaya 2 and Enrico Gratton 1*

1 University of Illinois
2 Northwestern University

* To whom correspondence should be addressed. E-mail: egratton22{at}yahoo.com.

Submitted on October 11, 2005
Revised on October 21, 2005
Accepted on 27 October 2005


  Abstract
We studied the motion of pigment organelles driven by myosin-V in Xenopus melanophores using a tracking technique with precision of 2 nm. The organelle trajectories showed occasional steps with a distribution centered at 35 nm and a standard deviation of 13 nm, in agreement with the step-size of myosin-V determined in vitro. In contrast, trajectories of melanosomes in cells expressing a dominant negative form of myosin-V did not show steps. The step duration was in the range 20-80 ms, slower than what it would be expected from in vitro results. We speculate that the cytoplasm high viscosity may affect significantly melanosomes motion

Key Words: actin, melanosome, myosin-V, particle tracking




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Copyright © 2005 by the Biophysical Society.