Self Assembly of Actin Scaffolds at Ponticulin Containing Supported Phospholipid Bilayers
Benjamin r. g. Johnson 1, Richard J Bushby 1, John Colyer 1 and Stephen Evans 1*
1 University of Leeds
* To whom correspondence should be addressed. E-mail: s.d.evans{at}leeds.ac.uk.
Submitted on October 21, 2005
Revised on November 4, 2005
Accepted on 7 November 2005
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Abstract |
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Phospholipid vesicles containing ponticulin have been used to form solid supported and tethered bilayer lipid membranes. The ponticulin serves as both a nucleation site for actin polymerization as well as a binding site for F-actin. Studies of F-actin binding to such bilayers have demonstrated the formation of an in-vitro actin scaffold. The dissociation constant for the binding of F-actin filaments to a ponticulin containing tethered bilayer was found to 11 ± 5 nM, indicative of high affinity binding.
Key Words:
Actin, Bilayer, Cytoskeleton, Membrane, Ponticulin, tethered
