help button home button Biophys. J.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Biophys. J. BioFAST: First Published April 7, 2006. doi:10.1529/biophysj.105.077180
© 2006 by the Biophysical Society.

A more recent version of this article appeared on July 1, 2006.
This Article
Right arrow Full Text (Rapid PDF)
Right arrow Supplemental
Right arrow All Versions of this Article:
biophysj.105.077180v1
91/1/1    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen-Izu, Y.
Right arrow Articles by Izu, L. T
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen-Izu, Y.
Right arrow Articles by Izu, L. T

BIOPHYSICAL THEORY AND MODELING

Three-Dimensional Distribution of Ryanodine Receptor Clusters in Cardiac Myocytes

Ye Chen-Izu 1, Stacey L McCulle 1, Chris W Ward 2, Christian Soeller 3, Bryan M Allen 4, Cal Rabang 5, Mark B Cannell 3, C William Balke 1 and Leighton T Izu 1*

1 University of Kentucky
2 University of Maryland Baltimore
3 Auckland University
4 Rochester Institute of Technology
5 University of Maryland at Baltimore County

* To whom correspondence should be addressed. E-mail: leightonizu{at}uky.edu.

Submitted on November 3, 2005
Revised on December 2, 2005
Accepted on 1 February 2006


  Abstract
The clustering of ryanodine receptors (RyR2) into functional Ca2+ release units is central to current models for cardiac excitation-contraction (E-C) coupling. Using immunolabeling and confocal microscopy, we have analyzed the distribution of RyR2 clusters in rat atrial and ventricular myocytes. The resolution of the 3-D structure was improved by a novel transverse sectioning method as well as digital deconvolution. In contrast to earlier reports, the mean RyR2 cluster transverse spacing was measured 1.05 µm in ventricular myocytes and estimated 0.97 µm in atrial myocytes. Intercalated RyR2 clusters were found interspersed between the Z-disks on the cell periphery but absent in the interior, forming double rows flanking the local Z-disks on the surface. The longitudinal spacing between the adjacent rows of RyR2 clusters on the Z-disks was measured to have a mean value of 1.87 µm in ventricular and 1.69 µm in atrial myocytes. The measured RyR2 cluster distribution is compatible with models of Ca2+ wave generation. The size of the typical RyR2 cluster was close to 250 nm, and this suggests that about 100 RyR2s might be present in a cluster. The importance of cluster size and 3-D spacing for current E-C coupling models is discussed.

Key Words: atrial myocyte, calcium signaling, calcium sparks, excitation-contraction coupling, ventricular myocyte




This article has been cited by other articles:


Home page
 Biophys. JHome page
J. G. Restrepo, J. N. Weiss, and A. Karma
Calsequestrin-Mediated Mechanism for Cellular Calcium Transient Alternans
Biophys. J., October 15, 2008; 95(8): 3767 - 3789.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
T. Tao, S. C. O'Neill, M. E. Diaz, Y. T. Li, D. A. Eisner, and H. Zhang
Alternans of cardiac calcium cycling in a cluster of ryanodine receptors: a simulation study
Am J Physiol Heart Circ Physiol, August 1, 2008; 295(2): H598 - H609.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
C. Picco, A. Naso, P. Soliani, and F. Gambale
The Zinc Binding Site of the Shaker Channel KDC1 from Daucus carota
Biophys. J., January 15, 2008; 94(2): 424 - 433.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
C. Orchard and F. Brette
t-tubules and sarcoplasmic reticulum function in cardiac ventricular myocytes
Cardiovasc Res, January 15, 2008; 77(2): 237 - 244.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
L. Chen, J. Zhang, T. X. Gan, Y. Chen-Izu, J. D. Hasday, M. Karmazyn, C. W. Balke, and S. M. Scharf
Left ventricular dysfunction and associated cellular injury in rats exposed to chronic intermittent hypoxia
J Appl Physiol, January 1, 2008; 104(1): 218 - 223.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
L. T. Izu, T. Banyasz, C. W. Balke, and Y. Chen-Izu
Eavesdropping on the Social Lives of Ca2+ Sparks
Biophys. J., November 15, 2007; 93(10): 3408 - 3420.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
P. Dan, E. Lin, J. Huang, P. Biln, and G. F. Tibbits
Three-Dimensional Distribution of Cardiac Na+-Ca2+ Exchanger and Ryanodine Receptor during Development
Biophys. J., October 1, 2007; 93(7): 2504 - 2518.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
V. Lukyanenko, A. Ziman, A. Lukyanenko, V. Salnikov, and W. J. Lederer
Functional groups of ryanodine receptors in rat ventricular cells
J. Physiol., August 15, 2007; 583(1): 251 - 269.
[Abstract] [Full Text] [PDF]

Home page
 Biophys. JHome page
G. S. B. Williams, M. A. Huertas, E. A. Sobie, M. S. Jafri, and G. D. Smith
A Probability Density Approach to Modeling Local Control of Calcium-Induced Calcium Release in Cardiac Myocytes
Biophys. J., April 1, 2007; 92(7): 2311 - 2328.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. D. Bootman, D. R. Higazi, S. Coombes, and H. L. Roderick
Calcium signalling during excitation-contraction coupling in mammalian atrial myocytes.
J. Cell Sci., October 1, 2006; 119(Pt 19): 3915 - 3925.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Copyright © 2006 by the Biophysical Society.