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Biophys. J. BioFAST: First Published January 6, 2006. doi:10.1529/biophysj.105.077941
© 2006 by the Biophysical Society.

A more recent version of this article appeared on March 1, 2006.
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BIOPHYSICAL LETTERS

Intracellular Transport Dynamics of Endosomes Containing DNA Polyplexes Along the Microtubule Network

Rajan P Kulkarni 1, Kenneth Castelino 2, Arun Majumdar 2 and Scott E Fraser 1*

1 Caltech
2 UC Berkeley

* To whom correspondence should be addressed. E-mail: sefraser{at}caltech.edu.

Submitted on November 16, 2005
Revised on December 4, 2005
Accepted on 4 January 2006


  Abstract
We have explored the transport of DNA polyplexes enclosed in endosomes within the cellular environment by multiple particle tracking (MPT). The polyplex-loaded endosomes demonstrate enhanced diffusion at short timescales (t < 7 sec) with their mean square displacement (MSD) <{Delta}x(t)2> scaling as t1.25. For longer time intervals they exhibit subdiffusive transport and have an MSD scaling as t0.7. This crossover from an enhanced diffusion to a subdiffusive regime can be explained by considering the action of motor proteins that actively transport these endosomes along the cellular microtubule network and the thermal bending modes of the microtubule network itself.

Key Words: Endosome transport, Microtubules, Multiple Particle Tracking, Polyplex, Transfection Agent






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Copyright © 2006 by the Biophysical Society.