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Biophys. J. BioFAST: First Published September 22, 2006. doi:10.1529/biophysj.106.089771
© 2006 by the Biophysical Society.

A more recent version of this article appeared on December 15, 2006.
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David Lasne
Gerhard A Blab
Stéphane Berciaud
Martin Heine
Laurent Groc
Daniel Choquet
Laurent Cognet
Brahim Lounis
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SPECTROSCOPY, IMAGING, OTHER TECHNIQUES

Single Nanoparticle Photothermal Tracking (SNaPT) of 5 nm gold beads in live cells

David Lasne 1, Gerhard A Blab 1, Stéphane Berciaud 1, Martin Heine 2, Laurent Groc 2, Daniel Choquet 2, Laurent Cognet 1 and Brahim Lounis 1*

1 CPMOH / CNRS-Université Bordeaux1
2 Institut F. Magendie / CNRS-Université Bordeaux2

* To whom correspondence should be addressed. E-mail: b.lounis{at}cpmoh.u-bordeaux1.fr.

Submitted on May 22, 2006
Revised on June 27, 2006
Accepted on 21 July 2006


  Abstract
Tracking individual nano-objets in live cells during arbitrary long times is an ubiquitous need in modern biology. We present here a method for tracking individual 5 nm gold nanoparticles on live cells. It relies on the photothermal effect and the detection of the Laser Induced Scattering around a NanoAbsorber (LISNA). The key point for recording trajectories at video rate is the use of a triangulation procedure. The effectiveness of the method is tested against Single fluorescent Molecule Tracking in live COS7 cells on subsecond time scales. We further demonstrate recordings for several minutes of AMPA receptors trajectories on the plasma membrane of live neurons. SNaPT has the unique potential to record arbitrary long trajectory of membrane proteins using non-fluorescent nanometer sized labels.

Key Words: Single molecule detection, membrane proteins diffusion, metal nanoparticle, photothermal microscopy, single particle tracking




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Copyright © 2006 by the Biophysical Society.