SPECTROSCOPY, IMAGING, OTHER TECHNIQUES |
Multiple Diffusion Mechanisms Due to Nanostructuring in Crowded Environments
Hugo Sanabria 1*, Yoshihisa Kubota 1 and M. Neal Waxham 1
1 The University of Texas Medical School at Houston
* To whom correspondence should be addressed. E-mail: hugo.sanabria{at}uth.tmc.edu.
Submitted on June 5, 2006
Revised on July 6, 2006
Accepted on 26 September 2006
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Abstract |
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One of the key questions regarding intracellular diffusion is how the environment affects molecular mobility. Mostly, intracellular diffusion has been described as hindered, and the physical reasons for this behavior are: immobile barriers, molecular crowding, and binding interactions with immobile or mobile molecules. Using results from multi-photon fluorescence correlation spectroscopy, we describe how immobile barriers and crowding agents affect translational mobility. To study the hindrance produced by immobile barriers, we used sol-gels (silica nanostructures) that consist of a continuous solid phase and aqueous phase in which fluorescently tagged molecules diffuse. In the case of molecular crowding, translational mobility was assessed in increasing concentrations of 500 kDa dextran solutions. Diffusion of fluorescent tracers in both sol-gels and dextran solutions shows clear evidence of anomalous subdiffusion. In addition, data from the autocorrelation function was analyzed using the maximum entropy method as adapted to fluorescence correlation spectroscopy data and compared with the standard model that incorporates anomalous diffusion. The maximum entropy method revealed evidence of different diffusion mechanisms that had not been revealed using the anomalous diffusion model. These mechanisms likely correspond to nanostructuring in crowded environments and to the relative dimensions of the crowding agent with respect to the tracer molecule. Analysis with the maximum entropy method also revealed information about the degree of heterogeneity in the environment as reported by the behavior of diffusive molecules.
Key Words:
Dextrans, Diffusion, Fluorescence correlation spectroscopy, Maximum entropy method, Molecular crowding, Sol-gels
