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Biophys. J. BioFAST: First Published October 6, 2006. doi:10.1529/biophysj.106.090969
© 2006 by the Biophysical Society.

A more recent version of this article appeared on January 1, 2007.
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CELL BIOPHYSICS

Transport governs flow-enhanced cell tethering through L-selectin at threshold shear

Tadayuki Yago 1, Veronika I Zarnitsyna 2, Arkadiusz G Klopocki 1, Rodger P McEver 1 and Cheng Zhu 2*

1 Oklahoma Medical Research Foundation
2 Georgia Institute of Technology

* To whom correspondence should be addressed. E-mail: cheng.zhu{at}me.gatech.edu.

Submitted on June 7, 2006
Revised on August 2, 2006
Accepted on 13 September 2006


  Abstract
Flow-enhanced cell adhesion is a counterintuitive phenomenon that has been observed in several biological systems. Flow augments L-selectin-dependent adhesion by increasing the initial tethering of leukocytes to vascular surfaces and by strengthening their subsequent rolling interactions. Tethering or rolling might be influenced by physical factors that affect the formation or dissociation of selectin-ligand bonds. We recently demonstrated that flow enhanced rolling of L-selectin-bearing microspheres or neutrophils on P-selectin glycoprotein ligand-1 (PSGL-1) by force that decreased bond dissociation. Here we show that flow augmented tethering of these microspheres or cells to PSGL-1 by three transport mechanisms that increased bond formation: sliding of the sphere bottom on the surface, Brownian motion, and molecular diffusion. These results elucidate the mechanisms for flow-enhanced tethering through L-selectin.

Key Words: Brownian motion, bond formation, cell adhesion, molecular diffusion, on-rate




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Copyright © 2006 by the Biophysical Society.