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Biophys. J. BioFAST: First Published November 10, 2006. doi:10.1529/biophysj.106.091884
© 2006 by the Biophysical Society.

A more recent version of this article appeared on February 1, 2007.
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CELL BIOPHYSICS

VASP governs actin dynamics by modulating filament anchoring

Léa Trichet 1, Otger Campàs 1, Cécile Sykes 2 and Julie Plastino 1*

1 Institut Curie
2 Institut Curie/CNRS

* To whom correspondence should be addressed. E-mail: julie.plastino{at}curie.fr.

Submitted on June 28, 2006
Revised on August 21, 2006
Accepted on 23 October 2006


  Abstract
Actin filament dynamics at the cell membrane are important for cell-matrix and cell-cell adhesions and the protrusion of the leading edge. Since actin filaments must be connected to the cell membrane to exert forces, but must also detach from the membrane to allow it to move and evolve, the balance between actin filament tethering and detachment at adhesion sites and the leading edge is key for cell shape changes and motility. How this fine-tuning is performed in cells remains an open question, but possible candidates are the Drosophila enabled/Vasodilator-stimulated phophoprotein (Ena/VASP) family of proteins, which localize to dynamic actin structures in the cell. Here we study VASP-mediated Arp2/3 complex-dependent actin dynamics using a substrate that mimics the fluid properties of the cell membrane: an oil-water interface. We show evidence that polymerization activators undergo diffusion and convection on the fluid surface, due to continual attachment and detachment to the actin network. These dynamics are enhanced in the presence of VASP, and we observe cycles of catastrophic detachment of the actin network from the surface, resulting in stop-and-go motion. These results point to a role for VASP in the modulation of filament anchoring, with implications for actin dynamics at cell adhesions and at the leading edge of the cell.

Key Words: Arp2/3, actin comet, adhesion, jumping motion, propulsion, protein mobility




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Copyright © 2006 by the Biophysical Society.