BIOPHYSICAL THEORY AND MODELING |
Actin polymerization upon processive capping by formin: a model for slowing and acceleration
Tom Shemesh 1 and Michael Kozlov 2*
1 Tel Aviv University
2 Tel Aviv University Sackler Faculty of Medicine
* To whom correspondence should be addressed. E-mail: michk{at}post.tau.ac.il.
Submitted on September 29, 2006
Revised on October 25, 2006
Accepted on 16 November 2006
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Abstract |
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Formin family proteins act as processive cappers of actin filaments, and determine the dynamics of a number of intracellular processes that are based on actin polymerization. The rate of filament growth upon processive capping varies within a broad range depending on the formin type and presence of profilin. While FH2 domains of various formins slow down polymerization by different extents, the FH1FH2 domains in conjunction with profilin accelerate the reaction. Study of the physical mechanism of processive capping is vital for understanding the intracellular actin dynamics. We propose a model predicting that variation of a single physical parameter - the effective elastic energy of the formin-capped barbed end - results in the observed diversity of the polymerization rates. The model accounts for the whole range of the experimental results including the drastic slowing down of polymerization by FH2 of Cdc12 formin and the 4.5 fold acceleration of the reaction by FH1-FH2 of mDai1 formin in the presence of profilin. Fitting the theoretical predictions to the experimental curves provides the values of the effective elastic energies of different formin-barbed end complexes.
Key Words:
Actin polymerization, cytoskeleton, dynamics, formin, kinetics, processive capping
