Label-free Calcium Imaging in Ischemic Retinal Tissue by TOF-SIMS

¹ Seoul National University
² Seoul National University Hospital
³ Korea Research Institute of Standards and Science

^* To whom correspondence should be addressed. E-mail: qwonkim{at}plaza.snu.ac.kr.

Submitted on August 21, 2007
Revised on September 29, 2007
Accepted on 8 November 2007

	Abstract

The distribution and movement of elemental ions in biologic tissues is critical for many cellular processes. In contrast to chemical techniques for imaging the intracellular distribution of ions, however, techniques for imaging the distribution of ions across tissues are not well developed. We used time-of-flight secondary ion mass spectrometry (TOF-SIMS) to obtain nonlabeled high-resolution analytic images of ion distribution in ischemic retinal tissues. Marked changes in Ca²⁺ distribution, compared with other fundamental ions, such as Na⁺, K⁺, and Mg²⁺, were detected during the progression of ischemia. Furthermore, the Ca²⁺ redistribution pattern correlated closely with TUNEL-positive (positive for terminal deoxynucleotidyl transferase-mediated dUTP [2'-deoxyuridine 5'-triphosphate] nick end-labeling) cell death in ischemic retinas. After treatment with a calcium chelator, Ca²⁺ ion redistribution was delayed, resulting in a decrease in TUNEL-positive cells. These results indicate that ischemia-induced Ca²⁺ redistribution within retinal tissues is associated with the order of apoptotic cell death, which possibly explains the different susceptibility of various types of retinal cells to ischemia. Thus, the TOF-SIMS technique provides a tool for the study of intercellular communication by Ca²⁺ ion movement.

Key Words: Calcium, Imaging, Retinal ischemia, TOF-SIMS