Kinetics of Multiplex Hybridization: Mechanisms and Implications
Justin Bishop 1, Alexander Chagovetz 1 and Steve Blair 1*
1 University of Utah
* To whom correspondence should be addressed. E-mail: blair{at}ece.utah.edu.
Submitted on September 6, 2007
Revised on October 8, 2007
Accepted on 18 October 2007
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Abstract |
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Quantitative analysis of DNA microarray data is complicated by uncertainties inherent to the experimental setup. Using computer simulations and real-time experimental results, we have previously demonstrated effects of multiplex reactions on a single sensing zone of an array, which may be a leading factor in erroneous interpretation of experimental data. We suggest here that a simplified three component kinetic model may present a sufficient approximation to describe the general case of DNA sensing in a complex sample milieu. We show that, by analyzing the real-time hybridization kinetics of a non-target species, we can perform quantitative analysis of unlabeled targets of interest within a broad dynamic range of concentrations.
Key Words:
DNA microarrays, kinteics, multiplex reactions
